Sequence: Not reported in paper
| Experiment Id | EXP000249 |
|---|---|
| Paper | Unraveling mRNA delivery bottlenecks of ineffective delivery vectors by co-transfection with effecti |
| Peptide | PepFect14 (PF14) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | very high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 2 µM |
| Rna Concentration | ~107–214 ng per well |
| Mixing Ratio | Electrostatic complexation |
| Formulation Format | Peptide–mRNA nanoparticles |
| Formulation Components | PF14 + mRNA |
| Size Nm | 42.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MC3T3-E1; HeLa; C5120 fibroblasts; hBMSC |
| Animal Model | |
| Administration Route | |
| Output Type | Protein expression (fluorescence & luciferase) |
| Output Value | Robust reporter expression |
| Output Units | |
| Output Notes | Efficient uptake and endosomal escape demonstrated |
| Toxicity Notes | Viability reduced ≥4 µM PF14 |
| Curation Notes |