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EXP000250

Paper

Unraveling mRNA delivery bottlenecks of ineffective delivery vectors by co-transfection with effective carriers (2024)

Peptide

Nona-arginine (R9)

Sequence: RRRRRRRRR

RNA

mRNA

All experiment fields

Experiment Id EXP000250
Paper Unraveling mRNA delivery bottlenecks of ineffective delivery vectors by co-transfection with effecti
Peptide Nona-arginine (R9)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence very high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration 1–5 µM
Rna Concentration ~107–214 ng per well
Mixing Ratio Electrostatic complexation
Formulation Format Peptide–mRNA nanoparticles
Formulation Components R9 + mRNA
Size Nm 86.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells MC3T3-E1; HeLa; C5120 fibroblasts; hBMSC
Animal Model
Administration Route
Output Type Protein expression
Output Value No expression unless co-transfected with PF14
Output Units
Output Notes Uptake occurs but endosomal escape insufficient
Toxicity Notes Low toxicity alone
Curation Notes