Back to browse

EXP000272

Paper

Endosomal escape cell-penetrating peptides significantly enhance pharmacological effectiveness and CNS activity of systemically administered antisense oligonucleotides (2021)

Peptide

HA2-ApoE(133–150)

Sequence: GLFHAIAHFIHGGWH-LRVRLASHLRKLRKRLLR

RNA

ASO (PMO)

All experiment fields

Experiment Id EXP000272
Paper Endosomal escape cell-penetrating peptides significantly enhance pharmacological effectiveness and C
Peptide HA2-ApoE(133–150)
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence very high
In Vitro Functional Effect yes
Endosomal Escape Evidence
Peptide Concentration 8 mg/kg
Rna Concentration Equimolar
Mixing Ratio 1:1 covalent conjugate
Formulation Format Peptide–PMO conjugate
Formulation Components HA2-ApoE(133–150)-PMO
Size Nm
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model SMN2 transgenic mice
Administration Route Intravenous (tail vein)
Output Type SMN2 exon-7 inclusion (RT-qPCR)
Output Value Significant increase in brain and spinal cord
Output Units
Output Notes Demonstrated BBB crossing and CNS delivery
Toxicity Notes Well tolerated at 8 mg/kg
Curation Notes