Sequence: KCFQWQRNMRKVRGPPVSCIKR
| Experiment Id | EXP000285 |
|---|---|
| Paper | Deciphering Structural Determinants Distinguishing Active from Inactive Cell-Penetrating Peptides fo |
| Peptide | hLF-WT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | N/P 3–5 |
| Rna Concentration | 107–214 ng/well |
| Mixing Ratio | Electrostatic polyplex (N/P) |
| Formulation Format | CPP–mRNA polyplex |
| Formulation Components | hLF-WT + mRNA |
| Size Nm | 2.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | MC3T3-E1; HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase or eGFP expression |
| Output Value | Measured |
| Output Units | |
| Output Notes | Only PF14 and hLF-v1 (polymeric) showed significant expression |
| Toxicity Notes | PF14 reduced metabolic activity at high N/P |
| Curation Notes |