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EXP000305

Paper

Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delivery of antisense oligomers (2011)

Peptide

Carrier CPP-PNA: Deca-cPNA1(9)-(D-Arg)8

Sequence: (D-Arg)8_SYS1

RNA

PNA (antisense)

All experiment fields

Experiment Id EXP000305
Paper Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delive
Peptide Carrier CPP-PNA: Deca-cPNA1(9)-(D-Arg)8
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence medium
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration
Mixing Ratio 1:1 (antisense PNA:carrier)
Formulation Format
Formulation Components Antisense PNA (naked asPNA) + carrier CPP-PNA Deca-cPNA1(9)-(D-Arg)8
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HeLa pLuc705
Animal Model
Administration Route
Output Type Luciferase splice-correction reporter activation
Output Value ≥20-fold activity increase at 6 μM antisense PNA with equimolar carrier (nonamer) (reported)
Output Units
Output Notes Naked antisense PNA alone inactive up to 6 μM; equimolar Deca-cPNA1(9)-(D-Arg)8 enhanced activity strongly (Fig.2A).
Toxicity Notes
Curation Notes