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EXP000306

Paper

Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delivery of antisense oligomers (2011)

Peptide

Antisense CPP-PNA: (D-Arg)8-asPNA + carrier cPNA1(7)-(D-Arg)8

Sequence: (D-Arg)8_SYS2

RNA

PNA (antisense)

All experiment fields

Experiment Id EXP000306
Paper Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delive
Peptide Antisense CPP-PNA: (D-Arg)8-asPNA + carrier cPNA1(7)-(D-Arg)8
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence medium
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration
Mixing Ratio 1:1 (antisense PNA:carrier)
Formulation Format
Formulation Components Antisense CPP-PNA (D-Arg)8-asPNA + carrier CPP-PNA cPNA1(7)-(D-Arg)8
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells HeLa pLuc705
Animal Model
Administration Route
Output Type Luciferase splice-correction reporter activation
Output Value ~6-fold improvement of (D-Arg)8-asPNA at 2 μM by heptamer carrier (reported)
Output Units
Output Notes Carrier CPP-PNA cPNA1(7)-(D-Arg)8 enhanced antisense CPP-PNA activity without significant extra toxicity; comparable to chloroquine enhancement at certain doses (Figs.3–4).
Toxicity Notes
Curation Notes