Antisense CPP-PNA: (D-Arg)8-asPNA + carrier Deca-cPNA1(6)-(D-Arg)8
Sequence: (D-Arg)8_SYS3
| Experiment Id | EXP000307 |
|---|---|
| Paper | Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delive |
| Peptide | Antisense CPP-PNA: (D-Arg)8-asPNA + carrier Deca-cPNA1(6)-(D-Arg)8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 1:1 (antisense PNA:carrier) |
| Formulation Format | |
| Formulation Components | Antisense CPP-PNA (D-Arg)8-asPNA + carrier CPP-PNA Deca-cPNA1(6)-(D-Arg)8 |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase splice-correction reporter activation |
| Output Value | ~8–10-fold improvement of (D-Arg)8-asPNA at 2 μM by hexamer decanoyl carrier (reported) |
| Output Units | |
| Output Notes | Deca-cPNA1(6)-(D-Arg)8 gave strongest enhancement among shorter carriers for CPP-asPNA (Fig.2B). Authors suggest carrier may facilitate endosomal escape (discussion comparing to CQ). |
| Toxicity Notes | |
| Curation Notes |