Antisense CPP-PNA: (D-Arg)8-asPNA + (Cholate)3-cPNA3(10) carrier
Sequence: (D-Arg)8_SYS4
| Experiment Id | EXP000308 |
|---|---|
| Paper | Peptide nucleic acid (PNA) cell penetrating peptide (CPP) conjugates as carriers for cellular delive |
| Peptide | Antisense CPP-PNA: (D-Arg)8-asPNA + (Cholate)3-cPNA3(10) carrier |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | |
| Mixing Ratio | 1:1 (antisense PNA:carrier) |
| Formulation Format | |
| Formulation Components | Antisense CPP-PNA (D-Arg)8-asPNA + (Cholate)3-cPNA3(10) carrier PNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705 |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase splice-correction reporter activation |
| Output Value | ~2-fold increase vs antisense CPP-PNA at 2 μM with (Cholate)3-cPNA3(10) (reported) |
| Output Units | |
| Output Notes | Cholic-acid modified carrier PNA modestly enhanced activity compared with CPP-carrier PNAs (Fig.5). |
| Toxicity Notes | |
| Curation Notes |