Delivery of siRNA complexed with palmitoylated α-peptide–β-peptoid cell-penetrating peptidomimetics (2015)
Sequence: α/β-peptoid chimera; X=Pam; n=4 (Fig.1)
| Experiment Id | EXP000322 |
|---|---|
| Paper | Delivery of siRNA complexed with palmitoylated α-peptide–β-peptoid cell-penetrating peptidomimetics |
| Peptide | Pam-α/β-peptoid-7 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | variable; optimized in study |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | nanocomplex |
| Formulation Components | Pam-α/β-peptoid-7 + siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa, U2OS |
| Animal Model | |
| Administration Route | |
| Output Type | reporter knockdown |
| Output Value | ~70% luciferase knockdown |
| Output Units | |
| Output Notes | Efficient cytosolic delivery; palmitoylation critical |
| Toxicity Notes | Low toxicity at effective doses |
| Curation Notes |