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EXP000327

Paper

Cell Penetrating Peptides Functionalized siRNA Nanoparticles Knockdown HER Receptors in Breast Cancer Cells (2025)

Peptide

cTAT

Sequence: c[CONH2-EYGRKKRRQRRRK-NH2]-Fmoc

RNA

siRNA

All experiment fields

Experiment Id EXP000327
Paper Cell Penetrating Peptides Functionalized siRNA Nanoparticles Knockdown HER Receptors in Breast Cance
Peptide cTAT
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 200–600 nM
Mixing Ratio
Formulation Format nanoparticle
Formulation Components methylated protamine + CPP + siRNA
Size Nm
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells BT474, SKBR3
Animal Model
Administration Route
Output Type gene knockdown
Output Value ~50–55% HER2 mRNA reduction
Output Units
Output Notes Moderate knockdown; improved uptake vs linear
Toxicity Notes >90% cell viability
Curation Notes