Polymer Nanoparticle-Mediated Delivery of MicroRNA Inhibition and Alternative Splicing (2012)
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| Experiment Id | EXP000348 |
|---|---|
| Paper | Polymer Nanoparticle-Mediated Delivery of MicroRNA Inhibition and Alternative Splicing |
| Peptide | ARG |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~15 μg peptide per mg NP |
| Rna Concentration | 1–2 μM |
| Mixing Ratio | Encapsulated (double emulsion) |
| Formulation Format | CPP-coated polymer nanoparticle |
| Formulation Components | PLGA NP + ARG CPP + PMO/PNA |
| Size Nm | 100.00 |
| Zeta Mv | -19.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | KB human oral carcinoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | miRNA inhibition; alternative splicing; apoptosis |
| Output Value | Reduced miR-155 levels; Mcl-1 exon skipping; reduced viability |
| Output Units | |
| Output Notes | Cytosolic and nuclear localization; strong functional antisense activity |
| Toxicity Notes | No significant toxicity for controls |
| Curation Notes |