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EXP000349

Paper

Efficient Liposomal Nanocarrier-mediated Oligodeoxynucleotide Delivery Involving Dual Use of a Cell-Penetrating Peptide as a Packaging and Intracellular Delivery Agent (2010)

Peptide

9R

Sequence: RRRRRRRRR

RNA

ODN

All experiment fields

Experiment Id EXP000349
Paper Efficient Liposomal Nanocarrier-mediated Oligodeoxynucleotide Delivery Involving Dual Use of a Cell-
Peptide 9R
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration Varied to achieve N/P ratios up to 1:6
Rna Concentration 0.5 μM ODN (cell studies)
Mixing Ratio 9R/ODN charge ratio 6:1
Formulation Format CPP-modified liposome
Formulation Components POPC/POPG/Chol/PEG-DSPE liposome + 9R/ODN polyplex
Size Nm 106.00
Zeta Mv
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells U87MG human glioblastoma cells
Animal Model
Administration Route
Output Type ODN uptake; NF-κB inhibition; chemosensitization; apoptosis
Output Value Enhanced paclitaxel sensitivity; increased apoptosis; reduced viability
Output Units
Output Notes Cytosolic delivery of ODN; prolonged intracellular retention; functional NF-κB inhibition demonstrated
Toxicity Notes Moderate cytotoxicity (~20% viability reduction) attributed to 9R/ODN core
Curation Notes