Rational modification of oligoarginine for highly efficient siRNA delivery (2015)
Sequence: Stearyl-HHHHHHHHRRRRRRRRRRRRRRR-NH2
| Experiment Id | EXP000355 |
|---|---|
| Paper | Rational modification of oligoarginine for highly efficient siRNA delivery |
| Peptide | STR-H8R15 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | Varied (R/P 2.5–10) |
| Rna Concentration | 100 nM (cell studies) |
| Mixing Ratio | R/P ratio 2.5–10 |
| Formulation Format | Peptide/siRNA nanoparticle |
| Formulation Components | Modified oligoarginine + siRNA |
| Size Nm | 200.00 |
| Zeta Mv | 20.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | CHO-K1; HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | Gene silencing (qPCR); uptake; endosomal escape assays |
| Output Value | Up to ~92% GAPDH knockdown (STR-H20R8) |
| Output Units | |
| Output Notes | Silencing correlates with His/Arg ratio; proton-sponge mediated escape |
| Toxicity Notes | Minimal cytotoxicity; lower than Lipofectamine |
| Curation Notes |