Sequence: LLRLLRWWWRLLRLL
| Experiment Id | EXP000365 |
|---|---|
| Paper | A short peptide for efficient cellular mRNA delivery: A potential application for inducing an immune |
| Peptide | WRAP5 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | very high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Defined by charge ratio (CR5) |
| Rna Concentration | 0.5–2 µg (in vitro); 5–15 µg (in vivo) |
| Mixing Ratio | Charge ratio 5 (positive peptide charges : RNA phosphates) |
| Formulation Format | Peptide-based nanoparticle |
| Formulation Components | WRAP5 ± 5% PEG-WRAP5 + mRNA in 5% glucose |
| Size Nm | 53.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vitro + in vivo |
| Cell Lines Or Primary Cells | HeLa cells |
| Animal Model | Mouse (OF1); Rabbit; Chick embryo |
| Administration Route | Intramuscular; intralimb (embryo) |
| Output Type | Protein expression; immune response |
| Output Value | Robust GFP expression in vitro; anti-GFP antibody production in vivo |
| Output Units | |
| Output Notes | Direct membrane translocation; long-term nanoparticle stability; repeated dosing |
| Toxicity Notes | Low toxicity at optimized dose |
| Curation Notes |