A self-accelerating endosomal escape siRNA delivery nanosystem for suppressing hyperplasia (2019)
Sequence: WGRRRRRRRRGC
| Experiment Id | EXP000373 |
|---|---|
| Paper | A self-accelerating endosomal escape siRNA delivery nanosystem for suppressing hyperplasia |
| Peptide | W-G-R8-G-C |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 400 nM |
| Mixing Ratio | 6:1 (+ coating) |
| Formulation Format | ternary nanoparticle |
| Formulation Components | POSS-(C-G-R8-G-W)16 / PLL-g-Aco / ERK2-siRNA |
| Size Nm | 100.00 |
| Zeta Mv | -1.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | VSMCs |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ERK2 mRNA ↓ ~50%, protein ↓, migration inhibited |
| Output Units | |
| Output Notes | Strong functional silencing in vitro |
| Toxicity Notes | Low cytotoxicity, good hemocompatibility |
| Curation Notes |