Peptide-Based Nanoparticles to Rapidly and Efficiently “Wrap ’n Roll” siRNA into Cells (2019)
Sequence: LLWRLWRLLWRLWRLL
| Experiment Id | EXP000379 |
|---|---|
| Paper | Peptide-Based Nanoparticles to Rapidly and Efficiently “Wrap ’n Roll” siRNA into Cells |
| Peptide | WRAP1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 400 nM |
| Rna Concentration | 20 nM |
| Mixing Ratio | R=20 |
| Formulation Format | peptide-based nanoparticle |
| Formulation Components | WRAP peptide / siRNA electrostatic NP |
| Size Nm | 80.00 |
| Zeta Mv | 25.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87, KB, MCF7, HuH7, HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | Luciferase and CDK4 knockdown (variable by peptide) |
| Output Units | |
| Output Notes | Screening and in vitro functional evaluation only |
| Toxicity Notes | Low cytotoxicity (<10%) |
| Curation Notes |