Sequence: Stearyl-AGYLLGKLARLARLARLAR (PF6; quinoline-modified Lys)
| Experiment Id | EXP000385 |
|---|---|
| Paper | Intracellular Delivery of Short Interfering RNA in Rat Organ of Corti Using a Cell-penetrating Pepti |
| Peptide | PepFect6 (PF6) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 2–4 µM |
| Rna Concentration | 50–200 nM |
| Mixing Ratio | 1:20 |
| Formulation Format | peptide/siRNA nanoparticle |
| Formulation Components | PepFect6 + siRNA (noncovalent) |
| Size Nm | |
| Zeta Mv | |
| Model Scope | ex_vivo |
| Model Type | ex vivo organotypic culture |
| Cell Lines Or Primary Cells | Rat organ of Corti explants |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown & functional impairment |
| Output Value | HPRT1, Cx26/Cx30 mRNA knockdown; reduced gap-junction communication |
| Output Units | |
| Output Notes | Ex vivo organotypic culture; no in vivo delivery |
| Toxicity Notes | No hair cell toxicity observed |
| Curation Notes |