Sequence: ALWKTLLKKVLKAPKKKRKVC
| Experiment Id | EXP000391 |
|---|---|
| Paper | Acylation of the S413-PV cell-penetrating peptide as a means of enhancing its capacity to mediate nu |
| Peptide | C14-S413-PV |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 0.44–2.2 µM |
| Rna Concentration | 50 nM |
| Mixing Ratio | (+/-)=2:1 |
| Formulation Format | peptide/siRNA electrostatic complex |
| Formulation Components | S413-PV derivative + siRNA |
| Size Nm | 100.00 |
| Zeta Mv | 20.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 glioblastoma cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene silencing |
| Output Value | GFP knockdown (variable; strongest for C12/C14) |
| Output Units | |
| Output Notes | In vitro only; no animal studies |
| Toxicity Notes | Low cytotoxicity at 2:1 ratio |
| Curation Notes |