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EXP000408

Paper

Development and characterization of chitosan-PEG-TAT nanoparticles for the intracellular delivery of siRNA (2013)

Peptide

CS-PEG-TAT/siRNA

Sequence: GRKKRRQRRRGC

RNA

siRNA

All experiment fields

Experiment Id EXP000408
Paper Development and characterization of chitosan-PEG-TAT nanoparticles for the intracellular delivery of
Peptide CS-PEG-TAT/siRNA
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect no
Endosomal Escape Evidence
Peptide Concentration
Rna Concentration 2 µg/mL
Mixing Ratio N/P ≈103
Formulation Format polymer-based nanoparticle
Formulation Components Chitosan-PEG-TAT polymer + siRNA
Size Nm 85.00
Zeta Mv 15.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells Mouse neuroblastoma Neuro2a cells
Animal Model
Administration Route
Output Type cellular uptake / transfection
Output Value High intracellular fluorescence; efficient uptake
Output Units
Output Notes In vitro nanoparticle optimization study
Toxicity Notes Low cytotoxicity at optimal pH
Curation Notes