Knockdown of a G protein-coupled receptor through efficient peptide-mediated siRNA delivery (2012)
Sequence: GLLEALAELLEK18FHTFPQTAIGVGAP-NH2|KKRKAPKKKRKFA
| Experiment Id | EXP000509 |
|---|---|
| Paper | Knockdown of a G protein-coupled receptor through efficient peptide-mediated siRNA delivery |
| Peptide | JH1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 8–17 µM |
| Rna Concentration | ~67 nM |
| Mixing Ratio | 125–250:1 (peptide:siRNA) |
| Formulation Format | CPP–siRNA electrostatic complex |
| Formulation Components | |
| Size Nm | 150.00 |
| Zeta Mv | 4.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HEK-293, MCF-7 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | |
| Output Units | |
| Output Notes | |
| Toxicity Notes | no significant cytotoxicity |
| Curation Notes |