Optimized luciferase assay for cell-penetrating peptide-mediated delivery of short oligonucleotides (2015)
Sequence: Stearyl-AGYLLGKLLOOLAAAALOOLL
| Experiment Id | EXP000511 |
|---|---|
| Paper | Optimized luciferase assay for cell-penetrating peptide-mediated delivery of short oligonucleotides |
| Peptide | PepFect14 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.75–1.2 µM |
| Rna Concentration | 40–150 nM |
| Mixing Ratio | 30:1 (siRNA) / 5:1 (SCO) |
| Formulation Format | CPP–oligonucleotide electrostatic complex |
| Formulation Components | |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705, U-87 MG-luc2 |
| Animal Model | |
| Administration Route | |
| Output Type | luciferase splice correction / knockdown |
| Output Value | >80% knockdown |
| Output Units | |
| Output Notes | Assay optimization study; PF14 used as positive control |
| Toxicity Notes | No significant toxicity at working concentrations |
| Curation Notes |