Sequence: CGRKKRWWPQRWWRWWRPPQ
| Experiment Id | EXP000512 |
|---|---|
| Paper | A Straightforward Method for the Development of Positively Charged Gold Nanoparticle-Based Vectors f |
| Peptide | Tat-modified CPP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | ≤15 µM non-toxic |
| Rna Concentration | 0.5–0.7 µg |
| Mixing Ratio | 1:40 (siRNA:AuNP, w/w) |
| Formulation Format | CPP-functionalized gold nanoparticles |
| Formulation Components | AuNP, cysteamine, CPP, siRNA |
| Size Nm | 20.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | SEAP-ARPE-19 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~50–75% SEAP reduction |
| Output Units | |
| Output Notes | Functional only when complexed to CPP-AuNPs |
| Toxicity Notes | Low toxicity at working concentrations |
| Curation Notes |