Sequence: Chol-HHHHHHH-AKRGARSTA
| Experiment Id | EXP000513 |
|---|---|
| Paper | Dual-Blockade Immune Checkpoint for Breast Cancer Treatment Based on a Tumor-Penetrating Peptide Ass |
| Peptide | CHL |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 25–160 nM |
| Mixing Ratio | N/P = 20 |
| Formulation Format | self-assembling peptide nanomicelle |
| Formulation Components | CHL, siPD-L1, ±1-methyl-DL-tryptophan |
| Size Nm | 175.00 |
| Zeta Mv | 20.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | 4T1 breast cancer cells |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown / immune activation |
| Output Value | |
| Output Units | |
| Output Notes | Efficient uptake and PD-L1 silencing; lysosomal escape via histidine block |
| Toxicity Notes | Low cytotoxicity |
| Curation Notes |