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EXP000514

Paper

Dual-Blockade Immune Checkpoint for Breast Cancer Treatment Based on a Tumor-Penetrating Peptide Assembling Nanoparticle (2019)

Peptide

CHL

Sequence: Chol-HHHHHHH-AKRGARSTA

RNA

siRNA

All experiment fields

Experiment Id EXP000514
Paper Dual-Blockade Immune Checkpoint for Breast Cancer Treatment Based on a Tumor-Penetrating Peptide Ass
Peptide CHL
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 14 mg/kg (siRNA equivalent)
Mixing Ratio N/P = 20
Formulation Format self-assembling peptide nanomicelle
Formulation Components CHL, siPD-L1, 1-methyl-DL-tryptophan
Size Nm 175.00
Zeta Mv 20.00
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model 4T1 breast cancer allograft (BALB/c mice)
Administration Route intravenous
Output Type tumor growth inhibition / immune activation
Output Value
Output Units
Output Notes Significant tumor suppression and increased CD8+/CD4+ ratio
Toxicity Notes No significant systemic toxicity
Curation Notes