NGR motif peptide + CPP (penetratin-derived)
Sequence: CYGGRGNG ; CKRRMKWKK
| Experiment Id | EXP000516 |
|---|---|
| Paper | Dual stimulus of hyperthermia and intracellular redox environment triggered release of siRNA for tum |
| Peptide | NGR motif peptide + CPP (penetratin-derived) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | Dose: 1.2 mg/kg siRNA (as Cy3-siRNA-CPPs formulations) for imaging and therapy studies |
| Mixing Ratio | |
| Formulation Format | Thermosensitive liposome (TSL) with NGR targeting; encapsulated siRNA-CPP conjugate; heat-triggered release |
| Formulation Components | DPPC:MSPC:DSPE-PEG2000-NGR (87:3:10, w/w); encapsulated siRNA-CPP conjugate; hyperthermia 42°C 30 min to trigger release/activate CPP; intracellular GSH reduces disulfide |
| Size Nm | 90.23 |
| Zeta Mv | -18.98 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Female BALB/c nude mice; HT-1080 xenograft tumor model |
| Administration Route | IV (tail vein); local hyperthermia 42°C for 30 min after each injection |
| Output Type | Tumor growth inhibition; in vivo c-myc knockdown (qRT-PCR/western); biodistribution imaging; TUNEL apoptosis |
| Output Value | Superior tumor accumulation, c-myc silencing, delayed tumor progression with hyperthermia |
| Output Units | |
| Output Notes | Compared against free siRNA-CPPs (under hyperthermia) and NGR-TSL without hyperthermia; NGR-TSL + hyperthermia showed strongest antitumor efficacy and gene silencing. |
| Toxicity Notes | No pronounced body weight loss reported for liposomal groups; free siRNA-CPPs caused body weight loss (off-target toxicity). |
| Curation Notes |