Sequence: CKRRMKWKK
| Experiment Id | EXP000517 |
|---|---|
| Paper | Dual stimulus of hyperthermia and intracellular redox environment triggered release of siRNA for tum |
| Peptide | CPP (penetratin-derived) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 250 nM (gene knockdown assays); 100 nM (uptake assays) |
| Mixing Ratio | |
| Formulation Format | Thermosensitive liposome (TSL) encapsulating siRNA-CPP conjugate; heat-triggered release |
| Formulation Components | DPPC:MSPC:DSPE-PEG2000 (87:3:10, w/w); encapsulated siRNA-CPP conjugate; hyperthermia 42°C 30 min (in vivo experiments); intracellular GSH reduces disulfide |
| Size Nm | 88.49 |
| Zeta Mv | -22.30 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT-1080 |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake (FACS/CLSM); gene knockdown (qRT-PCR / western) |
| Output Value | Only slight c-myc downregulation reported for unheated TSL formulation |
| Output Units | |
| Output Notes | In vitro comparisons primarily used unheated siRNA-CPPs/TSL; heat activation emphasized for NGR-TSL-H. |
| Toxicity Notes | |
| Curation Notes |