Sequence: CKRRMKWKK
| Experiment Id | EXP000518 |
|---|---|
| Paper | Dual stimulus of hyperthermia and intracellular redox environment triggered release of siRNA for tum |
| Peptide | CPP (penetratin-derived) |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | Dose: 1.2 mg/kg siRNA (as siRNA-CPPs/TSL formulations) |
| Mixing Ratio | |
| Formulation Format | Thermosensitive liposome (TSL) encapsulating siRNA-CPP conjugate; heat-triggered release |
| Formulation Components | DPPC:MSPC:DSPE-PEG2000 (87:3:10, w/w); encapsulated siRNA-CPP conjugate; hyperthermia 42°C 30 min (in vivo experiments); intracellular GSH reduces disulfide |
| Size Nm | 88.49 |
| Zeta Mv | -22.30 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Female BALB/c nude mice; HT-1080 xenograft |
| Administration Route | IV (tail vein) + hyperthermia 42°C 30 min |
| Output Type | Tumor growth inhibition; in vivo c-myc knockdown; biodistribution imaging |
| Output Value | Tumor growth inhibition and in vivo gene silencing under hyperthermia (less than NGR-TSL) |
| Output Units | |
| Output Notes | Hyperthermia increased tumor accumulation even without NGR; TSL group showed lower gene silencing than NGR-TSL but better than several controls. |
| Toxicity Notes | No pronounced body weight loss reported for liposomal groups |
| Curation Notes |