Sequence: CKRRMKWKK
| Experiment Id | EXP000519 |
|---|---|
| Paper | Dual stimulus of hyperthermia and intracellular redox environment triggered release of siRNA for tum |
| Peptide | CPP (penetratin-derived) |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 250 nM (gene knockdown assays); 100 nM (uptake assays) |
| Mixing Ratio | |
| Formulation Format | Covalent siRNA-CPP conjugate (no nanoparticle carrier) |
| Formulation Components | siRNA covalently conjugated to CPP via reducible disulfide bond; intracellular GSH reduces disulfide |
| Size Nm | |
| Zeta Mv | 11.25 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HT-1080 |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake (FACS/CLSM); gene knockdown (qRT-PCR / western) |
| Output Value | Efficient in vitro c-myc silencing reported for free siRNA-CPPs |
| Output Units | |
| Output Notes | Free siRNA-CPPs showed strong uptake; compared against free siRNA and liposomal formulations. |
| Toxicity Notes | |
| Curation Notes |