Sequence: CKRRMKWKK
| Experiment Id | EXP000520 |
|---|---|
| Paper | Dual stimulus of hyperthermia and intracellular redox environment triggered release of siRNA for tum |
| Peptide | CPP (penetratin-derived) |
| Delivery Success Class | no |
| In Vivo Flag | yes |
| Uptake Confirmed | no |
| Label Confidence | medium |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | Dose: 1.2 mg/kg (Cy3-siRNA-CPPs for imaging; c-myc siRNA-CPPs for therapy) |
| Mixing Ratio | |
| Formulation Format | Covalent siRNA-CPP conjugate (no nanoparticle carrier) |
| Formulation Components | siRNA covalently conjugated to CPP via reducible disulfide bond; intracellular GSH reduces disulfide |
| Size Nm | |
| Zeta Mv | 11.25 |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Female BALB/c nude mice; HT-1080 xenograft |
| Administration Route | IV (tail vein) + hyperthermia 42°C 30 min |
| Output Type | Tumor growth inhibition; in vivo c-myc knockdown; biodistribution imaging; body weight monitoring |
| Output Value | Less bioactivity in vivo vs NGR-TSL; nonspecific distribution; body weight loss observed |
| Output Units | |
| Output Notes | Authors report free siRNA-CPPs had efficient in vitro silencing but lower in vivo bioactivity, attributed to degradation/reduction in circulation. |
| Toxicity Notes | Significant body weight loss reported for free siRNA-CPPs group (off-target toxicity). |
| Curation Notes |