Novel cell penetrating peptides with multiple motifs composed of RGD and its analogs (2013)
Sequence: RGERGERGERLRERGERLC
| Experiment Id | EXP000524 |
|---|---|
| Paper | Novel cell penetrating peptides with multiple motifs composed of RGD and its analogs |
| Peptide | PE1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 0.6 µg siRNA per well (in vitro) |
| Mixing Ratio | |
| Formulation Format | Peptide-conjugated asymmetric liposome (ALP) |
| Formulation Components | DSPC, DOPE, mPEG-PE, miPEG-PE, cholesterol; siRNA encapsulated; peptide surface-conjugated |
| Size Nm | 250.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549, NCI-H322, NCI-H460, NIH-3T3, MDA-MB-435 |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake (confocal microscopy); gene silencing (GFP, TMPRSS4) |
| Output Value | Robust cellular uptake; significant in vitro gene silencing vs non-modified liposomes |
| Output Units | |
| Output Notes | All peptide-modified ALPs showed uptake; PD2 and PE2 showed strongest gene silencing |
| Toxicity Notes | PL cytotoxic at 100 µM; PD/PE peptides showed minimal toxicity |
| Curation Notes |