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EXP000542

Paper

A novel multitargeted self-assembling peptide-siRNA complex for simultaneous inhibition of SARS-CoV-2-host cell interaction and replication (2024)

Peptide

HE25

Sequence: stearyl-HEHHEHHEHEHGFLGRVRVLRGDKW-amide

RNA

siRNA

All experiment fields

Experiment Id EXP000542
Paper A novel multitargeted self-assembling peptide-siRNA complex for simultaneous inhibition of SARS-CoV-
Peptide HE25
Delivery Success Class no
In Vivo Flag yes
Uptake Confirmed no
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration
Rna Concentration 0.2–1 mg/kg
Mixing Ratio 1:40 or 1:100
Formulation Format Systemic peptide–siRNA complex
Formulation Components HE25–siRNA self-assembled complex
Size Nm 100.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model CIEA NOG mice; BALB/c mice
Administration Route IV (tail vein) and nose-to-lung inhalation
Output Type Biodistribution (SPECT/CT, confocal); lung gene silencing surrogate; safety
Output Value Strong lung accumulation; endothelial vs epithelial localization route-dependent; no weight loss or fibrosis
Output Units
Output Notes In vivo biodistribution demonstrated; no in vivo viral challenge efficacy study performed
Toxicity Notes No acute toxicity; no lung fibrosis or weight loss
Curation Notes