Enhancing Endosomal Escape for Intracellular Delivery of Macromolecular Biologic Therapeutics (2016)
Sequence: YGRKKRRQRRR-PEG6-GFWFG
| Experiment Id | EXP000544 |
|---|---|
| Paper | Enhancing Endosomal Escape for Intracellular Delivery of Macromolecular Biologic Therapeutics |
| Peptide | TAT-P6-GFWFG |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | 0–60 µM |
| Rna Concentration | |
| Mixing Ratio | |
| Formulation Format | Covalent peptide–cargo conjugate |
| Formulation Components | TAT CPP ± PEG6-spaced hydrophobic endosomal escape domain |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | H1299, MCF7, MDA-MB-231, HaCaT (GFPβ1-10 expressing) |
| Animal Model | |
| Administration Route | |
| Output Type | Cytosolic delivery (split-GFP complementation) |
| Output Value | Enhanced GFP fluorescence for EED-containing peptides vs TAT alone |
| Output Units | |
| Output Notes | Quantitative live-cell assay directly measures endosomal escape into cytoplasm |
| Toxicity Notes | High hydrophobicity variants showed cytotoxicity; PEG6 spacing reduced toxicity |
| Curation Notes |