Comparison of Cationic and Amphipathic Cell Penetrating Peptides for siRNA Delivery and Efficacy (2012)
Sequence: CKLALKLALKALKAALKLA
| Experiment Id | EXP000550 |
|---|---|
| Paper | Comparison of Cationic and Amphipathic Cell Penetrating Peptides for siRNA Delivery and Efficacy |
| Peptide | CMAP |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | ~60 µM (carrier CPP); 12.5 µM K21-PDP stock |
| Rna Concentration | 50–100 nM |
| Mixing Ratio | 2:1 N/P (K21-PDP:siRNA); 1:1 CPP:PDP for MAP or R6 |
| Formulation Format | CPP-conjugated oligolysine polyplex |
| Formulation Components | K21-PDP neutralized siRNA polyplex conjugated with CPP via reducible disulfide bond |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | Huh7.5; HeLa |
| Animal Model | |
| Administration Route | |
| Output Type | Cellular uptake (fluorescence); gene silencing (GFP, PTEN) |
| Output Value | MAP-polyplex showed high uptake and silencing; R6-polyplex minimal; controls inactive |
| Output Units | |
| Output Notes | Ac-R4, Ac-R6, Ac-R8 tested for displacement only; functional delivery demonstrated only for MAP-polyplex |
| Toxicity Notes | Minimal toxicity for all CPP-polyplexes at 50 nM siRNA |
| Curation Notes |