Identification and Characterization of Receptor-Specific Peptides for siRNA Delivery (2012)
Sequence: DAATATRGRSAASRPTERPRAPARSASRPRRPVDGGGGK(TAMRA)CGNKRTRGC
| Experiment Id | EXP000560 |
|---|---|
| Paper | Identification and Characterization of Receptor-Specific Peptides for siRNA Delivery |
| Peptide | VP22 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | ~2 µM |
| Rna Concentration | 50–100 nM |
| Mixing Ratio | ~20:1 peptide:siRNA |
| Formulation Format | Noncovalent peptide–siRNA nanocomplex |
| Formulation Components | Tandem CPP–LyP-1 peptides condensing siRNA |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; OVCAR-8; MDA-MB-435 |
| Animal Model | |
| Administration Route | |
| Output Type | Cellular uptake; GFP knockdown; endosomal escape |
| Output Value | Subset achieved >50% GFP knockdown with receptor specificity |
| Output Units | |
| Output Notes | Best performer: myr-TP-LyP-1; polyarginines showed uptake but reduced specificity |
| Toxicity Notes | 12R and 15R showed reduced viability |
| Curation Notes |