A Cell-Penetrating Helical Polymer for siRNA Delivery to Mammalian Cells (2012)
Sequence: Poly(G-(4-vinylbenzyl)-L-glutamate) grafted with aminoethyl piperidine (degree of polymerization varies)
| Experiment Id | EXP000571 |
|---|---|
| Paper | A Cell-Penetrating Helical Polymer for siRNA Delivery to Mammalian Cells |
| Peptide | PVBLG150-8 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | |
| Rna Concentration | 25–100 nM |
| Mixing Ratio | 1:1–10:1 polymer:siRNA (w/w) |
| Formulation Format | Polymer–siRNA nanocomplex |
| Formulation Components | PVBLGn-8 electrostatically complexed with siRNA |
| Size Nm | 150.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa-Luc |
| Animal Model | |
| Administration Route | |
| Output Type | Luciferase knockdown; uptake; membrane permeation |
| Output Value | Up to ~80% luciferase knockdown (best for PVBLG100-8) |
| Output Units | |
| Output Notes | Delivery occurs via direct membrane pore formation, independent of endocytosis or endosomal acidification |
| Toxicity Notes | Lower toxicity than 25 kDa PEI; hemolysis at high concentrations |
| Curation Notes |