Sequence: YGRKKRRQRRR
| Experiment Id | EXP000581 |
|---|---|
| Paper | Effective siRNA delivery and target mRNA degradation using an amphipathic peptide to facilitate pH-d |
| Peptide | TAT |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | no |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 50 μg/mL (screening) |
| Rna Concentration | 20–50 nM |
| Mixing Ratio | ~40–280:1 peptide:siRNA (molar) |
| Formulation Format | Noncovalent peptide–siRNA co-incubation |
| Formulation Components | CPP mixed with siRNA prior to cell exposure |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; Huh-7; HEK293T |
| Animal Model | |
| Administration Route | |
| Output Type | Target mRNA knockdown (bDNA assay) |
| Output Value | Robust (~75–80%) knockdown only for Endoporter |
| Output Units | |
| Output Notes | Other CPPs showed uptake but no functional siRNA delivery |
| Toxicity Notes | No significant toxicity for any peptide at tested doses |
| Curation Notes |