Sequence: RRRRRRRRRGGGG
| Experiment Id | EXP000592 |
|---|---|
| Paper | The spacer arm length in cell-penetrating peptides influences chitosan/siRNA nanoparticle delivery f |
| Peptide | R9G4 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | Defined by degree of substitution (~2 peptides per chitosan chain) |
| Rna Concentration | 100 pmol/mL (in vitro); 1 μg per mouse (in vivo) |
| Mixing Ratio | Chitosan/siRNA wt/wt = 50 |
| Formulation Format | Oligopeptide-modified chitosan/siRNA nanoparticles |
| Formulation Components | Chitosan backbone covalently grafted with R9Gn peptides |
| Size Nm | 170.00 |
| Zeta Mv | 7.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 |
| Animal Model | |
| Administration Route | |
| Output Type | Uptake and gene silencing screening |
| Output Value | Moderate uptake and limited knockdown |
| Output Units | |
| Output Notes | Screened variant; not optimal for in vivo delivery |
| Toxicity Notes | Low toxicity in vitro and in vivo |
| Curation Notes |