Sequence: KRRRRRRRR
| Experiment Id | EXP000601 |
|---|---|
| Paper | Conjugates of HA2 with octaarginine-grafted HPMA copolymer offer effective siRNA delivery and gene s |
| Peptide | P-(R8)9.5-FITC |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 500 nM |
| Mixing Ratio | N/P=6 |
| Formulation Format | polyion complex (PIC) / polyplex |
| Formulation Components | HPMA copolymer + grafted K-R8 (9.5 mol%) + FITC; complexed with (Cy5-)RAC1 siRNA |
| Size Nm | 169.90 |
| Zeta Mv | 18.40 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | SK-OV-3 (confocal uptake); A549 (qRT-PCR) |
| Animal Model | |
| Administration Route | |
| Output Type | uptake / complexation |
| Output Value | |
| Output Units | |
| Output Notes | Efficient cellular delivery of Cy5-siRNA into SK-OV-3 cells (confocal). Despite uptake, RAC1 mRNA knockdown was ineffective without endosomal escape aid (endosomal entrapment hypothesized). |
| Toxicity Notes | Low in vitro cytotoxicity reported up to 1000 nM siRNA (MTT). |
| Curation Notes |