Sequence: KRRRRRRRR
| Experiment Id | EXP000602 |
|---|---|
| Paper | Conjugates of HA2 with octaarginine-grafted HPMA copolymer offer effective siRNA delivery and gene s |
| Peptide | P-(R8)9.5-FITC + chloroquine |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | medium |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 500 nM |
| Mixing Ratio | N/P=6 |
| Formulation Format | polyion complex (PIC) / polyplex + endosomal escape adjuvant |
| Formulation Components | HPMA copolymer + grafted K-R8 (9.5 mol%) + FITC; complexed with RAC1 siRNA; chloroquine added during transfection |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | RAC1 mRNA decreased (qRT-PCR; CQ-dependent) |
| Output Units | |
| Output Notes | Chloroquine co-treatment enabled significant RAC1 mRNA reduction versus P-(R8)9.5-FITC without CQ, supporting endosomal entrapment otherwise. |
| Toxicity Notes | CQ used transiently (6 h); polymer/siRNA complex itself reported low toxicity. |
| Curation Notes |