Sequence: KRRRRRRRR + GLFGAIAGFIENGWEGMIDGK
| Experiment Id | EXP000603 |
|---|---|
| Paper | Conjugates of HA2 with octaarginine-grafted HPMA copolymer offer effective siRNA delivery and gene s |
| Peptide | P-(R8)8.6-(HA2)7.6-FITC |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 500 nM |
| Mixing Ratio | N/P=8 |
| Formulation Format | polyion complex (PIC) / polyplex with fusogenic peptide |
| Formulation Components | HPMA copolymer + grafted K-R8 + HA2 fusogenic peptide + FITC; complexed with RAC1 siRNA |
| Size Nm | 196.80 |
| Zeta Mv | 18.20 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | A549 (qRT-PCR); SK-OV-3 (tox) |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~40% RAC1 silencing at 500 nM (A549) |
| Output Units | |
| Output Notes | HA2-bearing system achieved significantly higher gene silencing versus P-(R8)9.5-FITC, attributed to improved endosomal escape. |
| Toxicity Notes | Low in vitro cytotoxicity reported (MTT) in SK-OV-3 and A549. |
| Curation Notes |