Deciphering the internalization mechanism of WRAP:siRNA nanoparticles (2020)
Sequence: LLWRLWRLLWRLWRLL
| Experiment Id | EXP000604 |
|---|---|
| Paper | Deciphering the internalization mechanism of WRAP:siRNA nanoparticles |
| Peptide | WRAP1 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | variable (R=20) |
| Rna Concentration | 20–50 nM |
| Mixing Ratio | 20:1 (WRAP:siRNA) |
| Formulation Format | peptide-based nanoparticle |
| Formulation Components | WRAP1 + siRNA |
| Size Nm | 80.00 |
| Zeta Mv | 30.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | U87 glioblastoma, MEF TKO |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~80–90% knockdown |
| Output Units | |
| Output Notes | Efficient luciferase silencing; uptake via mixed mechanisms |
| Toxicity Notes | No significant toxicity |
| Curation Notes |