Engineered PepFect14 analog for efficient cellular delivery of oligonucleotides (2025)
Sequence: Stearoyl-AGYLLGKLLKKLAAAALKKLL-NH2
| Experiment Id | EXP000617 |
|---|---|
| Paper | Engineered PepFect14 analog for efficient cellular delivery of oligonucleotides |
| Peptide | PF14-Lys |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | MR28:1 |
| Rna Concentration | 60 pmol per injection |
| Mixing Ratio | MR28:1 |
| Formulation Format | CPP–miRNA nanoparticles |
| Formulation Components | PF14-Lys |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Mouse irritant contact dermatitis model |
| Administration Route | subcutaneous (ear) |
| Output Type | phenotypic rescue / gene suppression |
| Output Value | Reduced ear swelling; ↓Tnfa, Cxcl1, Icam1 |
| Output Units | |
| Output Notes | Functional in vivo miRNA delivery demonstrated |
| Toxicity Notes | Well tolerated |
| Curation Notes |