Engineered PepFect14 analog for efficient cellular delivery of oligonucleotides (2025)
Sequence: Stearoyl-AGYLLGRLLOOLAAAALOOLL-NH2
| Experiment Id | EXP000619 |
|---|---|
| Paper | Engineered PepFect14 analog for efficient cellular delivery of oligonucleotides |
| Peptide | PF14-R7 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | no |
| Endosomal Escape Evidence | |
| Peptide Concentration | 0.5–1 µM |
| Rna Concentration | 15–200 nM |
| Mixing Ratio | MR5:1 (SCO); MR17–34:1 (siRNA) |
| Formulation Format | self-assembled CPP–ON nanoparticles |
| Formulation Components | PF14-R7 |
| Size Nm | 20.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa pLuc705, HeLa EGFP654, U87 MG-Luc2, primary keratinocytes |
| Animal Model | |
| Administration Route | |
| Output Type | splice correction / gene knockdown |
| Output Value | up to ~200-fold reporter change |
| Output Units | |
| Output Notes | α-helicity required; Ca2+ enhances efficacy |
| Toxicity Notes | Low toxicity |
| Curation Notes |