Tumor suppressor protein-inspired peptide for siRNA delivery and synergistic cancer therapy (2025)
Sequence: CPP44-p16MIS-4R (CPP44RPM)
| Experiment Id | EXP000622 |
|---|---|
| Paper | Tumor suppressor protein-inspired peptide for siRNA delivery and synergistic cancer therapy |
| Peptide | CPP44 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | CPP44RPM:siRNA = 10:1 (molar) |
| Rna Concentration | 100 nM |
| Mixing Ratio | PEG-PEI:CPP44RPM:siRNA = 20:10:1 |
| Formulation Format | peptide–polymer–siRNA nanoparticles (PPCi) |
| Formulation Components | CPP44RPM + PEG-PEI + siPLK1 |
| Size Nm | 110.00 |
| Zeta Mv | 1.00 |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HepG2, THP-1 |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown / apoptosis |
| Output Value | ~50% PLK1 mRNA reduction; ~60% apoptosis |
| Output Units | |
| Output Notes | Efficient uptake and endosomal escape; synergistic apoptosis via p16MIS |
| Toxicity Notes | Acceptable in vitro toxicity |
| Curation Notes |