WRAP-based nanoparticles for siRNA delivery in zebrafish embryos by simple bath immersion (2025)
Sequence: LLRLLRWWWRLLRLL
| Experiment Id | EXP000625 |
|---|---|
| Paper | WRAP-based nanoparticles for siRNA delivery in zebrafish embryos by simple bath immersion |
| Peptide | WRAP5 |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | MR 20:1 |
| Rna Concentration | 40–60 nM |
| Mixing Ratio | 20:1 (peptide:siRNA) |
| Formulation Format | CPP–siRNA nanoparticles |
| Formulation Components | WRAP5:siRNA |
| Size Nm | 80.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HEK293-GFP |
| Animal Model | |
| Administration Route | |
| Output Type | gene knockdown |
| Output Value | ~50% GFP reduction |
| Output Units | |
| Output Notes | Efficient GFP silencing; no cytotoxicity |
| Toxicity Notes | No significant toxicity |
| Curation Notes |