WRAP-based nanoparticles for siRNA delivery in zebrafish embryos by simple bath immersion (2025)
Sequence: LLWRLWRLLWRLWRLL
| Experiment Id | EXP000626 |
|---|---|
| Paper | WRAP-based nanoparticles for siRNA delivery in zebrafish embryos by simple bath immersion |
| Peptide | WRAP1 |
| Delivery Success Class | yes |
| In Vivo Flag | yes |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | |
| Endosomal Escape Evidence | |
| Peptide Concentration | MR 20:1 |
| Rna Concentration | 35–140 nM |
| Mixing Ratio | 20:1 |
| Formulation Format | PEGylated CPP–siRNA nanoparticles |
| Formulation Components | 20% PEG-WRAP1:siRNA |
| Size Nm | 85.00 |
| Zeta Mv | |
| Model Scope | in_vivo |
| Model Type | in vivo |
| Cell Lines Or Primary Cells | |
| Animal Model | Zebrafish embryos Tg[fli1:EGFP]y1 |
| Administration Route | bath immersion |
| Output Type | in vivo gene knockdown |
| Output Value | 60–80% GFP silencing |
| Output Units | |
| Output Notes | Dose-dependent GFP knockdown by western blot and confocal microscopy |
| Toxicity Notes | No developmental toxicity |
| Curation Notes |