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EXP000627

Paper

Tumor microenvironment-activated cancer cell membrane-liposome hybrid nanoparticle-mediated synergistic metabolic therapy and chemotherapy for NSCLC (2021)

Peptide

MMP9-switchable CPP (R9-PVGLIG-EGGEGGEGG)

Sequence: RRRRRRRRR-PVGLIG-EGGEGGEGG

RNA

siRNA

All experiment fields

Experiment Id EXP000627
Paper Tumor microenvironment-activated cancer cell membrane-liposome hybrid nanoparticle-mediated synergis
Peptide MMP9-switchable CPP (R9-PVGLIG-EGGEGGEGG)
Delivery Success Class no
In Vivo Flag no
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect yes
Endosomal Escape Evidence yes
Peptide Concentration N/P = 4
Rna Concentration 20 nM
Mixing Ratio N/P 4
Formulation Format Hybrid biomimetic nanoparticles (CLip-PC@CO-LC)
Formulation Components Cancer cell membrane + liposome + CPP + LC + siRNA + DTX
Size Nm 193.00
Zeta Mv -33.00
Model Scope in_vitro
Model Type in vitro
Cell Lines Or Primary Cells A549 (NSCLC)
Animal Model
Administration Route
Output Type gene knockdown + antiproliferation
Output Value Strong PGAM1 knockdown; apoptosis
Output Units
Output Notes Synergistic siRNA + DTX effect; MMP-9 dependent uptake
Toxicity Notes Low cytotoxicity (blank NPs)
Curation Notes