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EXP000630

Paper

Systemic siRNA delivery to tumors by cell-penetrating α-helical polypeptide-based metastable nanoparticles (2018)

Peptide

PVBLG-8

Sequence: polypeptide (exact sequence not disclosed)

RNA

siRNA

All experiment fields

Experiment Id EXP000630
Paper Systemic siRNA delivery to tumors by cell-penetrating α-helical polypeptide-based metastable nanopar
Peptide PVBLG-8
Delivery Success Class yes
In Vivo Flag yes
Uptake Confirmed yes
Label Confidence high
In Vitro Functional Effect
Endosomal Escape Evidence yes
Peptide Concentration 1 mg siRNA/kg
Rna Concentration 1 mg/kg
Mixing Ratio as above
Formulation Format metastable polymeric nanoparticles (PSPP NPs)
Formulation Components PVBLG-8 + PLG + siRNA
Size Nm 120.00
Zeta Mv
Model Scope in_vivo
Model Type in vivo
Cell Lines Or Primary Cells
Animal Model U-87 MG xenograft nude mice
Administration Route intravenous
Output Type tumor growth inhibition / gene silencing
Output Value significant tumor suppression; strong EGFR knockdown
Output Units
Output Notes Superior to PLL and Lipofectamine 2000
Toxicity Notes No significant systemic toxicity
Curation Notes