Design of a new cell penetrating peptide for DNA, siRNA and mRNA delivery (2022)
Sequence: GLGTLLTLLHFLLHHLLHFLKRKRQQ-CONH2
| Experiment Id | EXP000635 |
|---|---|
| Paper | Design of a new cell penetrating peptide for DNA, siRNA and mRNA delivery |
| Peptide | HELP-4H |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | yes |
| Peptide Concentration | varied (w/w ratios) |
| Rna Concentration | 50 nM siRNA; 0.3–0.75 µg DNA/mRNA |
| Mixing Ratio | w/w 5–20 |
| Formulation Format | CPP–nucleic acid complexes |
| Formulation Components | HELP-4H + nucleic acid |
| Size Nm | 100.00 |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HEK293, A549, HCT116, MRC5-V2 |
| Animal Model | |
| Administration Route | |
| Output Type | gene expression / knockdown |
| Output Value | robust reporter expression or silencing |
| Output Units | |
| Output Notes | HELP-4H most efficient; serum-tolerant |
| Toxicity Notes | Low to moderate at high doses |
| Curation Notes |