Sequence: HHHHHALWKTLLKKVLKAPKKKRKVC-NH2
| Experiment Id | EXP000647 |
|---|---|
| Paper | Comparison of the Efficiency of Complexes Based on S413-PV Cell-Penetrating Peptides in Plasmid DNA |
| Peptide | H5-S413-PV |
| Delivery Success Class | no |
| In Vivo Flag | no |
| Uptake Confirmed | yes |
| Label Confidence | high |
| In Vitro Functional Effect | yes |
| Endosomal Escape Evidence | |
| Peptide Concentration | |
| Rna Concentration | 50 nM (siRNA); 1 µg/mL (pDNA) |
| Mixing Ratio | varied charge ratios (2/1–10/1) |
| Formulation Format | binary and ternary electrostatic complexes |
| Formulation Components | CPP ± DOTAP:DOPE liposomes + nucleic acid |
| Size Nm | |
| Zeta Mv | |
| Model Scope | in_vitro |
| Model Type | in vitro |
| Cell Lines Or Primary Cells | HeLa; A549; HT1080 |
| Animal Model | |
| Administration Route | |
| Output Type | reporter expression / gene knockdown |
| Output Value | Up to ~69% GFP knockdown (H5-S413-PV); survivin mRNA knockdown up to ~70% (cell-dependent) |
| Output Units | |
| Output Notes | Extensive in vitro comparison of uptake, knockdown, toxicity; no in vivo studies. |
| Toxicity Notes | Reverse NLS peptide showed high toxicity; H5-S413-PV lowest toxicity. |
| Curation Notes |